proteome profiler mouse xl 21 cytokine array kit  (R&D Systems)

Journal: bioRxiv

Article Title: NETosis and Myeloperoxidase Promotes Inflammation and Cardiac Remodeling in Arrhythmogenic Cardiomyopathy

doi: 10.64898/2026.04.14.718596

Figure Lengend Snippet: (A) Representative Western blots clearly show the upregulation of PAD4 and H3Cit in 4-week-old Dsg2 mut/mut cardiac lysates. (B) Quantification of western blots from . Data presented as mean±SEM; n=4 mice/parameter; ***P<0.001 and ****P<0.0001 via two-tailed T-test. (C) Representative ECG tracings from WT, Dsg2 mut/mut , and Dsg2 mut/mut ; Pad4 -/- at 16-weeks of age, respectively. (D) Percent ectopic beats at 16-weeks of age. Data presented as mean±SEM; n≥7. Statistical significance was determined via one-way ANOVA with Tukey’s multiple comparison test (**P<0.01 and ***P<0.001). (E) Percent left ventricular ejection fraction at 16-weeks of age. (F) Percent left ventricular fractional shortening at 16-weeks of age. (G) Stroke Volume at 16-weeks of age. (H) Representative Masson’s trichrome-immunostained hearts from Dsg2 mut/mut and Dsg2 mut/mut ; Pad4 -/- at 16-weeks of age. (I) Percent fibrosis at 16-weeks of age. Data presented as mean±SEM; n≥12. Statistical significance was determined via one-way ANOVA with Tukey’s multiple comparison test for E-G and via two-tailed T-test for I (*P<0.05, ***P<0.001, and ****P<0.0001). (J) Representative cytokine arrays with legend for key cytokines from WT, Dsg2 mut/mut , and Dsg2 mut/mut ; Pad4 -/- mice. (K) Legend of selected cytokines. (L-N) Quantification of selected cytokines normalized to reference band in WT, Dsg2 mut/mut , and Dsg2 mut/mut ; Pad4 -/- mice. Data presented as mean±SEM; n=4. *P<0.05, via two-tailed T-test. C5/C5a, Complement Component C5/C5a; MCP-1, Monocyte Chemoattractant Protein-1; MIP-α/β, Macrophage Inflammatory Protein-1 alpha/beta; MMP-3, Matrix metalloproteinase-3; MMP-9, Matrix metalloproteinase-9; MPO, Myeloperoxidase; NGAL, Neutrophil gelatinase-associated lipocalin; TNFα, Tumor Necrosis Factor-alpha; and OPN, Osteopontin.

Article Snippet: Cardiac cytokine profiling was conducted using the Proteome Profiler Mouse XL Cytokine Array Kit (R&D Systems, Cat. No. ARY028) as previously described.

Techniques: Western Blot, Two Tailed Test, Comparison

Journal: bioRxiv

Article Title: NETosis and Myeloperoxidase Promotes Inflammation and Cardiac Remodeling in Arrhythmogenic Cardiomyopathy

doi: 10.64898/2026.04.14.718596

Figure Lengend Snippet: (A) Schematic timeline of PF1355 treatment and in vivo mouse measurements. (B) Percent left ventricular ejection fraction (%LVEF) at 16-weeks of age. (C) Percent left ventricular fractional shortening (%FS) at 16-weeks of age. Data presented as mean±SEM; n≥5. Statistical significance was determined via two-way ANOVA with Tukey’s multiple comparison test (*P<0.05 and **P<0.01). (D) Representative ECG tracings from PF1355 treated Dsg2 mut/mut , Placebo treated Dsg2 mut/mut , and WT at 16-weeks of age, respectively. Asterisks, ectopic beats. (E) Percent ectopic beats (% Ectopics), (F) R-amplitude, and (G) Q-amplitude at 16-weeks of age. (H) Representative Masson’s trichrome-immunostained hearts from Placebo-treated and PF1355-treated Dsg2 mut/mut at 16-weeks of age. (I) Percent fibrosis at 16-weeks of age. Data presented as mean±SEM; n≥6. Statistical significance was determined via Welch’s t-test for E and one-way ANOVA with Tukey’s multiple comparison test for F,G, and I (*P<0.05). (J) Representative cytokine arrays with legend for key cytokines from Placebo-treated and PF1355-treated Dsg2 mut/mut , and WT mice. (K) Legend of selected cytokines. (L-N) Quantification of selected cytokines normalized to reference band in PF1355-treated and Placebo-treated Dsg2 mut/mut , and WT mice. Data presented as mean±SEM; n=4. *P<0.05, via two-tailed T-test. C5/C5a, Complement Component C5/C5a; CCL11, C-C motif chemokine ligand 11; CCL12, C-C motif chemokine ligand 12; LIX, Lipopolysaccharide-induced CXC chemokine; MCP-1, Monocyte Chemoattractant Protein-1; MIP-α/β, Macrophage Inflammatory Protein-1 alpha/beta; MMP-3, Matrix metalloproteinase-3; MMP-9, Matrix metalloproteinase-9; and NGAL, Neutrophil gelatinase-associated lipocalin. All data presented as mean±SEM. Statistical significance was determined via two-way ANOVA with Tukey’s multiple comparison test for B-C and via two-tailed T-test for E-G, I, and L-N (*P<0.05).

Article Snippet: Cardiac cytokine profiling was conducted using the Proteome Profiler Mouse XL Cytokine Array Kit (R&D Systems, Cat. No. ARY028) as previously described.

Techniques: In Vivo, Comparison, Two Tailed Test

Journal: Journal of Virology

Article Title: SARS-CoV-2 spike protein expression drives post-acute coagulopathy

doi: 10.1128/jvi.01255-25

Figure Lengend Snippet: IGFBP-1 and CXCL13 are potential biomarkers for coagulopathy induced by the S protein and for identifying long COVID patients. ( A ) Cytokine expression in plasma from hACE2 mice infected with VSVdG/G or Rep-S Delta R682G viruses (high dose:1 × 10 8 pfu/mouse) at 3 and 8–10 dpi was analyzed using a cytokine array. The changes in cytokine expression were calculated and presented in a heatmap, with the top 10 cytokines showing the greatest increase marked. ( B–C ) Plasma levels of CXCL13 and IGFBP-1 in infected mice (high dose: 2 × 10 7 pfu/mouse) were measured by ELISA (one-way ANOVA and Pearson correlation, * P < 0.05, ** P < 0.01, and *** P < 0.001). ( D ) Cytokine expression in lung tissue from hACE2 mice infected with VSVdG/G or Rep-S Delta R682G (1 × 10 8 pfu/mouse) at 9–11 dpi was profiled by cytokine array. Detailed cytokine levels in plasma and lung samples are provided in the supplementary data. ( E–F ) Serum levels of IGFBP-1 and CXCL13 were measured by ELISA in individuals without prior COVID-19 infection ( n = 15) and in long COVID patients ( n = 80). The non-COVID-19 group included individuals diagnosed with hepatic adenoma, focal nodular hyperplasia, or hemangioma. (Mann–Whitney U test, * P < 0.05).

Article Snippet: Cytokine detection in the mouse plasma and lung sample was performed using the Mouse XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA; ARY028), following the manufacturer’s instructions.

Techniques: Expressing, Clinical Proteomics, Infection, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY